d2d3 88 274  (Cell Signaling Technology Inc)

Journal: International Journal of Molecular Sciences

Article Title: Distinct Signaling Cascades Elicited by Different Formyl Peptide Receptor 2 (FPR2) Agonists

doi: 10.3390/ijms14047193

Figure Lengend Snippet: Intracellular signaling cascades triggered by peptides associated with inflammatory and anti-inflammatory responses.

Article Snippet: Pretreatment of monocytes with the FPR2 agonist, D2D3 88–274 , markedly decreases chemokine-induced integrin-dependent rapid cell adhesion [ ], indicating that FPR2 regulates leukocyte chemotaxis as a direct mediator of cell migration and/or by suppressing cell responses to chemokines by desensitizing chemokine receptors [ ]. uPAR works in concert with co-receptors, including integrins, FPR2 and epidermal growth factor receptor (EGFR), which may be dynamically organized into a multiprotein signaling receptor complex to initiate cell signaling [ ].

Techniques: Chemotaxis Assay, Expressing, Inhibition, Activity Assay, Phospho-proteomics, Translocation Assay, Activation Assay, Migration

Journal:

Article Title: The fibrinolytic receptor for urokinase activates the G protein-coupled chemotactic receptor FPRL1/LXA4R

doi: 10.1073/pnas.022652999

Figure Lengend Snippet: MMK-1 peptide desensitizes THP-1 cells to D2D3 88–274

Article Snippet: 125 I-D2D3 88–274 (5 nM) (Iodogen, Pierce), specific activity 30 μCi/μg, was incubated with 1.5–2 × 10 6 cells in 100 μl of binding buffer (RPMI medium 1640, 0.5% BSA) for 30 min at room temperature in the presence or absence of increasing concentrations of unlabeled D2D3 88–274 or other competitors.

Techniques:

Journal:

Article Title: The fibrinolytic receptor for urokinase activates the G protein-coupled chemotactic receptor FPRL1/LXA4R

doi: 10.1073/pnas.022652999

Figure Lengend Snippet: Effect of pretreatment of HEK293 FPRL1/LXA4R cells with activated uPAR on the chemotactic response to fMLP

Article Snippet: 125 I-D2D3 88–274 (5 nM) (Iodogen, Pierce), specific activity 30 μCi/μg, was incubated with 1.5–2 × 10 6 cells in 100 μl of binding buffer (RPMI medium 1640, 0.5% BSA) for 30 min at room temperature in the presence or absence of increasing concentrations of unlabeled D2D3 88–274 or other competitors.

Techniques: Migration

Journal:

Article Title: The fibrinolytic receptor for urokinase activates the G protein-coupled chemotactic receptor FPRL1/LXA4R

doi: 10.1073/pnas.022652999

Figure Lengend Snippet: Binding specificity of iodinated D2D3 88–274 on HEK293/FPRL1–LXA4R cells

Article Snippet: 125 I-D2D3 88–274 (5 nM) (Iodogen, Pierce), specific activity 30 μCi/μg, was incubated with 1.5–2 × 10 6 cells in 100 μl of binding buffer (RPMI medium 1640, 0.5% BSA) for 30 min at room temperature in the presence or absence of increasing concentrations of unlabeled D2D3 88–274 or other competitors.

Techniques: Binding Assay